Charecterization of mutation induced in Fenugreek by Gamma Ray using rapd marker

This study was accomplished to detect variation generated through eposure of fenugreek (trigonella foenum-graecum L.) seeds seven gamma irradiation doses (0,25,50,100,150,300 and 400) GY by using twenty RAPDs primers as molecular marker ,results indicated that OPA-14 ,OPC-19 OPH-01success in giving five treatments a unique fingerprint. Molecular size amplicon ranged between 2913 bp produced primer OPB-06 118 OPW-04.Highest degree for polymorphism 90.92% OPC-05. Monomorphic bands appeared their higher value OPC-08. Primer OPA-15 gave highest number OPH-01 (amplified, main) bands, discrimination, addition efficiency value. Silght produce among OPE-02, OPC-05, OPA-14. revealed RAPD markers detection mutation induced irradiation.